pmd2 g plasmid Search Results


98
Addgene inc vesicular stomatitis virus envelope expression plasmid pmd2 g
Vesicular Stomatitis Virus Envelope Expression Plasmid Pmd2 G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genechem lentivirus packaging plasmids pspax2
Lentivirus Packaging Plasmids Pspax2, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SignaGen helper plasmids pmd2g
Helper Plasmids Pmd2g, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vigene Biosciences packaging plasmids pmd2g
Packaging Plasmids Pmd2g, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NovoPro Biosciences Inc pmdlg/prre, pmd2.g, prsv- rev plasmids
Pmdlg/Prre, Pmd2.G, Prsv Rev Plasmids, supplied by NovoPro Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beyotime plasmid pmd2g
Plasmid Pmd2g, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Applied Biological Materials Inc mir-92a-3p lentivirus expression vector
<t>miR-92a-3p</t> expression in RCC tissues and cell lines. (A) The mean level of miR-92a-3p expression in RCC tissues was significantly elevated compared with matched non-tumor tissues, as determined by RT-qPCR. *P<0.05 vs. non-tumor tissues (n=16). (B) RT-qPCR was used to compare the differences in miR-92a-3p expression level between the non-tumorigenic renal cell line HK-2 and RCC cell lines ACHN and SN12PM6. *P<0.05 vs. HK-2 (n=3). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Mir 92a 3p Lentivirus Expression Vector, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Procell Inc pmd2.g envelope plasmid
<t>miR-92a-3p</t> expression in RCC tissues and cell lines. (A) The mean level of miR-92a-3p expression in RCC tissues was significantly elevated compared with matched non-tumor tissues, as determined by RT-qPCR. *P<0.05 vs. non-tumor tissues (n=16). (B) RT-qPCR was used to compare the differences in miR-92a-3p expression level between the non-tumorigenic renal cell line HK-2 and RCC cell lines ACHN and SN12PM6. *P<0.05 vs. HK-2 (n=3). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Pmd2.G Envelope Plasmid, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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VGTI Florida envelope plasmid pmd2g
<t>miR-92a-3p</t> expression in RCC tissues and cell lines. (A) The mean level of miR-92a-3p expression in RCC tissues was significantly elevated compared with matched non-tumor tissues, as determined by RT-qPCR. *P<0.05 vs. non-tumor tissues (n=16). (B) RT-qPCR was used to compare the differences in miR-92a-3p expression level between the non-tumorigenic renal cell line HK-2 and RCC cell lines ACHN and SN12PM6. *P<0.05 vs. HK-2 (n=3). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Envelope Plasmid Pmd2g, supplied by VGTI Florida, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABclonal Biotechnology pmd2.g envelope plasmids
<t>miR-92a-3p</t> expression in RCC tissues and cell lines. (A) The mean level of miR-92a-3p expression in RCC tissues was significantly elevated compared with matched non-tumor tissues, as determined by RT-qPCR. *P<0.05 vs. non-tumor tissues (n=16). (B) RT-qPCR was used to compare the differences in miR-92a-3p expression level between the non-tumorigenic renal cell line HK-2 and RCC cell lines ACHN and SN12PM6. *P<0.05 vs. HK-2 (n=3). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Pmd2.G Envelope Plasmids, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Johns Hopkins HealthCare plasmid psm4019/pmd2.g
<t>miR-92a-3p</t> expression in RCC tissues and cell lines. (A) The mean level of miR-92a-3p expression in RCC tissues was significantly elevated compared with matched non-tumor tissues, as determined by RT-qPCR. *P<0.05 vs. non-tumor tissues (n=16). (B) RT-qPCR was used to compare the differences in miR-92a-3p expression level between the non-tumorigenic renal cell line HK-2 and RCC cell lines ACHN and SN12PM6. *P<0.05 vs. HK-2 (n=3). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Plasmid Psm4019/Pmd2.G, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PlasmidFactory gmbh pmd2g envelope plasmid
<t>miR-92a-3p</t> expression in RCC tissues and cell lines. (A) The mean level of miR-92a-3p expression in RCC tissues was significantly elevated compared with matched non-tumor tissues, as determined by RT-qPCR. *P<0.05 vs. non-tumor tissues (n=16). (B) RT-qPCR was used to compare the differences in miR-92a-3p expression level between the non-tumorigenic renal cell line HK-2 and RCC cell lines ACHN and SN12PM6. *P<0.05 vs. HK-2 (n=3). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Pmd2g Envelope Plasmid, supplied by PlasmidFactory gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miR-92a-3p expression in RCC tissues and cell lines. (A) The mean level of miR-92a-3p expression in RCC tissues was significantly elevated compared with matched non-tumor tissues, as determined by RT-qPCR. *P<0.05 vs. non-tumor tissues (n=16). (B) RT-qPCR was used to compare the differences in miR-92a-3p expression level between the non-tumorigenic renal cell line HK-2 and RCC cell lines ACHN and SN12PM6. *P<0.05 vs. HK-2 (n=3). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Journal: Oncology Letters

Article Title: Cell proliferation is induced in renal cell carcinoma through miR-92a-3p upregulation by targeting FBXW7

doi: 10.3892/ol.2020.11443

Figure Lengend Snippet: miR-92a-3p expression in RCC tissues and cell lines. (A) The mean level of miR-92a-3p expression in RCC tissues was significantly elevated compared with matched non-tumor tissues, as determined by RT-qPCR. *P<0.05 vs. non-tumor tissues (n=16). (B) RT-qPCR was used to compare the differences in miR-92a-3p expression level between the non-tumorigenic renal cell line HK-2 and RCC cell lines ACHN and SN12PM6. *P<0.05 vs. HK-2 (n=3). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Article Snippet: The miR-92a-3p lentivirus expression vector and inhibitor hsa-miR-92b-3p lentivector (anti-miR-92a-3p) were purchased from Applied Biological Materials Inc. and were used to stably overexpress or knock down miR-92a-3p, respectively, in ACHN and SN12PM6 cell lines.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Biological effect of miR-92a-3p overexpression on ACHN and SN12PM6 cells. (A) RCC cell lines ACHN and SN12PM6 stably overexpressed miR-92a-3p following miR-92a-3p transfection. *P<0.05 vs. untransfected cells (blank); ^ P<0.05 vs. miR-control (n=3). (B) miR-92a-3p was stably downregulated in RCC cell lines ACHN and SN12PM6 following anti-miR-92a-3p transfection. *P<0.05 vs. untransfected cells (blank); ^ P<0.05 vs. anti-miR-control (n=3). (C) miR-92a-3p overexpression stimulated ACHN and SN12PM6 cell proliferation. *P<0.05 vs. untransfected cells; ^ P<0.05 vs. miR-control (n=3). (D) Colony formation assay demonstrated that miR-92a-3p-overexpression and FBXW7-knockdown accelerated colony formation of ACHN and SN12PM6 cells. Conversely, miR-92a-3p knockdown and FBXW7 overexpression inhibited colony formation of ACHN and SN12PM6 cells. (E) Number of colony forming cells/well expressed as percentage of untransfected cells (blank) and presented as the mean ± standard error. *P<0.05 vs. blank. (F) miR-92a-3p knockdown inhibited ACHN and SN12PM6 cell proliferation. *P<0.05 vs. untransfected cells, ^P<0.05 vs. anti-miR-control (n=3). FBXW7, F-box and WD repeat domain containing 7; RCC, renal cell carcinoma. (G and H) Western blotting determination of Cdc42 protein expression level in ACHN and SN12PM6 cells following miR-92a-3p or anti-miR-92a-3p transfection. FBXW7, F-box and WD repeat domain containing 7; RCC, renal cell carcinoma.

Journal: Oncology Letters

Article Title: Cell proliferation is induced in renal cell carcinoma through miR-92a-3p upregulation by targeting FBXW7

doi: 10.3892/ol.2020.11443

Figure Lengend Snippet: Biological effect of miR-92a-3p overexpression on ACHN and SN12PM6 cells. (A) RCC cell lines ACHN and SN12PM6 stably overexpressed miR-92a-3p following miR-92a-3p transfection. *P<0.05 vs. untransfected cells (blank); ^ P<0.05 vs. miR-control (n=3). (B) miR-92a-3p was stably downregulated in RCC cell lines ACHN and SN12PM6 following anti-miR-92a-3p transfection. *P<0.05 vs. untransfected cells (blank); ^ P<0.05 vs. anti-miR-control (n=3). (C) miR-92a-3p overexpression stimulated ACHN and SN12PM6 cell proliferation. *P<0.05 vs. untransfected cells; ^ P<0.05 vs. miR-control (n=3). (D) Colony formation assay demonstrated that miR-92a-3p-overexpression and FBXW7-knockdown accelerated colony formation of ACHN and SN12PM6 cells. Conversely, miR-92a-3p knockdown and FBXW7 overexpression inhibited colony formation of ACHN and SN12PM6 cells. (E) Number of colony forming cells/well expressed as percentage of untransfected cells (blank) and presented as the mean ± standard error. *P<0.05 vs. blank. (F) miR-92a-3p knockdown inhibited ACHN and SN12PM6 cell proliferation. *P<0.05 vs. untransfected cells, ^P<0.05 vs. anti-miR-control (n=3). FBXW7, F-box and WD repeat domain containing 7; RCC, renal cell carcinoma. (G and H) Western blotting determination of Cdc42 protein expression level in ACHN and SN12PM6 cells following miR-92a-3p or anti-miR-92a-3p transfection. FBXW7, F-box and WD repeat domain containing 7; RCC, renal cell carcinoma.

Article Snippet: The miR-92a-3p lentivirus expression vector and inhibitor hsa-miR-92b-3p lentivector (anti-miR-92a-3p) were purchased from Applied Biological Materials Inc. and were used to stably overexpress or knock down miR-92a-3p, respectively, in ACHN and SN12PM6 cell lines.

Techniques: Over Expression, Stable Transfection, Transfection, Colony Assay, Western Blot, Expressing

FBWX7 was directly suppressed by miR-92a-3p. (A) 3′-UTR luciferase reporter assay was used to confirm that miR-92a-3p bound to specific regions in the FBXW7 3′-UTR region. *P<0.05 vs. blank; ^ P<0.05 vs. miR-control (n=3). (B) FBXW7 mRNA level was assessed following miR-92a-3p overexpression by RT-qPCR. *P<0.05 vs. blank; ^ P<0.05 vs. miR-control (n=3). (C) Western blotting of FBXW7 in ACHN and SN12PM6 cells not transfected with lentivirus (blank) or transfected with lentivirus expressing miR-92a-3p or miR-control. (D) Western blotting bands were quantified using ImageJ software. Relative FBXW7 expression levels were normalized to β-actin. *P<0.05 vs. blank; ^ P<0.05 vs. miR-control (n=3). (E) FBXW7 mRNA level was measured following anti-miR-92a-3p overexpression in ACHN and SN12PM6 cell lines by RT-qPCR. *P<0.05 vs. blank; ^ P<0.05 vs. anti-miR-control (n=3). (F) FBXW7 protein expression was determined by western blotting in ACHN and SN12PM6 cell lines not transfected with lentivirus (blank) or transfected with a lentivirus expressing anti-miR-92a-3p or anti-miR-control. (G) Western blotting bands were quantified using ImageJ software. Relative FBXW7 expression levels were normalized to β-actin. *P<0.05 vs. blank, ^ P<0.05 vs. anti-miR-control (n=3). FBXW7, F-box and WD repeat domain containing 7; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Journal: Oncology Letters

Article Title: Cell proliferation is induced in renal cell carcinoma through miR-92a-3p upregulation by targeting FBXW7

doi: 10.3892/ol.2020.11443

Figure Lengend Snippet: FBWX7 was directly suppressed by miR-92a-3p. (A) 3′-UTR luciferase reporter assay was used to confirm that miR-92a-3p bound to specific regions in the FBXW7 3′-UTR region. *P<0.05 vs. blank; ^ P<0.05 vs. miR-control (n=3). (B) FBXW7 mRNA level was assessed following miR-92a-3p overexpression by RT-qPCR. *P<0.05 vs. blank; ^ P<0.05 vs. miR-control (n=3). (C) Western blotting of FBXW7 in ACHN and SN12PM6 cells not transfected with lentivirus (blank) or transfected with lentivirus expressing miR-92a-3p or miR-control. (D) Western blotting bands were quantified using ImageJ software. Relative FBXW7 expression levels were normalized to β-actin. *P<0.05 vs. blank; ^ P<0.05 vs. miR-control (n=3). (E) FBXW7 mRNA level was measured following anti-miR-92a-3p overexpression in ACHN and SN12PM6 cell lines by RT-qPCR. *P<0.05 vs. blank; ^ P<0.05 vs. anti-miR-control (n=3). (F) FBXW7 protein expression was determined by western blotting in ACHN and SN12PM6 cell lines not transfected with lentivirus (blank) or transfected with a lentivirus expressing anti-miR-92a-3p or anti-miR-control. (G) Western blotting bands were quantified using ImageJ software. Relative FBXW7 expression levels were normalized to β-actin. *P<0.05 vs. blank, ^ P<0.05 vs. anti-miR-control (n=3). FBXW7, F-box and WD repeat domain containing 7; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Article Snippet: The miR-92a-3p lentivirus expression vector and inhibitor hsa-miR-92b-3p lentivector (anti-miR-92a-3p) were purchased from Applied Biological Materials Inc. and were used to stably overexpress or knock down miR-92a-3p, respectively, in ACHN and SN12PM6 cell lines.

Techniques: Luciferase, Reporter Assay, Over Expression, Quantitative RT-PCR, Western Blot, Transfection, Expressing, Software, Real-time Polymerase Chain Reaction